WAYS TO OPTIMIZE THE THERAPY OF CORONAVIRUS INFECTION CAUSED BY COVID -19

Abstract

After electrophoresis, the results of 7 separate PCR reactions for each SARS-CoV-2 strain were converted into a binary matrix for subsequent in silico analysis. Positive amplification results were recorded as “1”, and negative ones as “0”, indicating the presence or absence of a specific DFR locus in a particular representative of SARS-CoV-2. Thus, the treatment of coronavirus infection with a new combination drug based on G. Lucidum and black cumin is a pathogenetically substantiated method.
Primers with a high content of G+C form a stronger bond with the template, which means that their ability to bind to partially non-complementary DNA regions increases. This should lead to the amplification of a larger number of fragments and an increase in the differentiating ability of the RAPD method. Thus, the detection and elimination of coronavirus infection caused by SARS CoV-2 is achieved after the use of a new drug based on G. Lucidum and black cumin (95% CI = 1.3-5.6 at χ2 = 0.9321007, U (Mann- Winnie) = 0.8721093, H (Kruskes-Wallis test) = 0.9102385 at p≤0.05